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Vestibulo-Ocular Reflex Recordings of Small Rodents using a Novel
Marker Array
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Mi Joo Kim, Jiyeon Lee, Eui Jae Hong, Eun Ji Lee, Yu Jin Min, Dong Ju Lee, Nam Beom Kim, Gyu Cheol Han
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Res Vestib Sci. 2016;15(1):11-16.
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 Abstract
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- Objective: Recording the nystagmus of small experimental rodents is an integral
technique in vestibular research. Theoretically, the size and the shape of markers
strongly affect the analysis of 3 dimensional nystagmus.
Methods
The nystagmus of 6 healthy ICR mice were recorded and their gain
values were compared using 200 μm, 300 μm, 400 μm, and 600 μm isosceles
triangle markers at the peak velocity of 60o/sec and 100o/sec with the rotational
stimulations of 0.1 Hz, 0.2 Hz, and 0.5 Hz.
Results
The gain values of 3 different sizes of the markers showed no significant
differences in horizontal- vertical-torsional component. However, it was unable
to record the nystagmus with 200 μm markers since the markers were too small
to be placed and stayed on the center of the pupils.
Conclusion
Technicians can decide the size of the markers from 200 to 600 ?m
to record the nystagmus of mice, depending on the technicians’ skills.
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Quantification of Vestibulospinal Reflex Under the Stress Condition on Both Soleus Muscles
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Mi Joo Kim, Seo Jin Jang, Eun Ji Lee, Ah Ram Yu, Jong Hyun Hwang, Gyu Cheol Han, Ju Kang Lee
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Res Vestib Sci. 2012;11(1):14-22.
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Abstract
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- Therefore, by establishing method that enables to track and observe quantified VSR, the reliability of experiment is attempted to be increased. Materials and Methods:On five SD Rats to measure electromyogram (EMG), electrodes, in the form of stranded cables composed of seven wires, are chronically implanted on both sides of the soleus muscles. Pre and post operative condition of sitting still, standing, and ladder step walking was compared before, and six and twenty four hours after a unilateral labyrinthectomy with quantified muscle activity in maximal voluntary activity. Simultaneously by using multi modality electric potential plus package, the muscle activity between the two legs was tracked and compared. Results: In the sitting still position, the left/right soleus muscle activities were 25.7/26.0 μV before a unilateral labyrinthectomy which was changed after the surgery with the value of 23.1/8.1 μV and 23.4/14.3 μV when six and twenty four hours passed respectively. In the standing position, 92.8/124.0 μV of preoperative value was changed to 89.6/37.3 μV six hours after the unilateral labyrinthectomy, and it was 97.0/54.7 μV 24 hours after. The preoperative value in ladder step walking test was 56.2/86.0 μV, and postoperative ones were 54.9/21.2 μV and 55.7/38.0 μV after six and twenty four hours respectively. Conclusion: VSR assessment method by using quantitative EMG well reflects the process of vestibular compensation, and to maintain the tension of extensor muscles, ladder step walking test is shown to be useful.
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C-fos Expression of Vestibular Nucleus in C57BL/6 Mouse Under the 7 Tesla Ultra High Magnetic Field
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Mi Joo Kim, Min Joo Jung, Ji Hye Bae, Eun Ji Lee, Duk Hyun Kim, Gyu Cheol Han
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Res Vestib Sci. 2011;10(1):26-29.
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Abstract
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- Background and Objectives: A typical side effect of super high magnetic field is dizziness and it is expected that 7 Tesla (T) super high magnetic field will have temporary influence on vestibular nucleus. Prevalence rate of dizziness generally increases with advancing years, so this study discusses whether the influence of 7 T super high magnetic field differs depending on the age.
Materials and Methods: C57BL/6 mice weighting from 10-40 g were categorized into 4-week-old and 16-week-old groups. They were exposed to 7 T magnetic field and immunohistochemical staining was done to observe expression of c-fos protein in vestibular nucleus of mice.
Results: Control group with normal vestibular system did not show c-fos protein. However, mice exposed to 7 T magnetic field showed c-fos expression selectively on medial and lateral parts of vestibular nucleus and there was no significant difference of c-fos expression between both sides (p<0.05). There was statistically significant difference between the different age group: 16-week-old mice group showed higher number of cells expressing c-fos protein than 4-week-old mice group did (p<0.05).
Conclusion: It is statistically significant that 7 T super high magnetic field is more influential to older age group.
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